USW
Systems & Synthetic BiologyGenetic CircuitsMediumSeed

Tune a genetic toggle switch for robust bistability

Choose promoter/RBS strengths so a synthetic toggle is bistable across conditions.

Dr. Ravi Menon
Bioengineering Dept.
Robust design of synthetic gene circuits
Nature Communications · 2022
~3 h
registered 2025-05-18
circuitbistabilityrobustness

End goal

Maintain bistability across ≥ 90% of sampled parameter perturbations.

Overview

The agent parameterizes a toggle-switch ODE model, searches the part-strength space, and reports a design with a wide bistable region robust to parameter noise.

Tools allowed

1
COBRApy (FBA)·Terminal

Workflow

1-step protocol

Each step is verified against the scientist's targets. Open any simulation to test it live.

  1. 1

    Search part strengths

    Step 1 / 1

    Search promoter/RBS strengths for a robust bistable region.

    Protocol

    1. aDefine the ODE model.
    2. bSweep part strengths.
    3. cScore robustness.

    Targets

    Robust bistability≥90% perturbations
    Expected output

    A robust toggle-switch parameter set.